Abstract: Fluorescein fundus angiography ( FFA) can accurately and intuitively reflect fundus-related lesions, and is indispensable in the detection of fundus disease animal models. The quality of FFA images is mainly affected by the appearing intensity and appearing time, which are controlled by many factors. The author combines many years of FFA experience in various species of experimental animals and refers to domestic and foreign research literature, summarize and analyze various controllable factors that affect the appearing intensity and time of FFA. We focus on comparing the differences in blood viscosity, blood pH, body temperature of different species of experimental animals and human, as well as the appearing intensity of FFA and the initial time, maintenance time and subside time. The differences of FFA among experimental animal species and the factors causing animal adverse reactions during FFA were analyzed. We hope the article which providing the information and data can help solve the problems encountered by researchers in FFA of experimental animals, obtain more accurate and reliable detection result, and realize the application value of FFA detection in experimental animals.

Key words: FFA, developing intensity, initial development time, subsiding time, development time, experimental animals

摘要： 荧光素眼底血管造影( FFA)可以精准直观的反应眼底相关病变,在眼底疾病动物模型的检测中不可或缺。FFA 图片质量主要受显影强度和显影时间的影响,而显影强度和显影时间受诸多因素调控。 笔者结合实验动物不同物种多年 FFA 拍摄经验并参考国内外研究文献,总结分析影响实验动物 FFA 显影强度和时间的各项可控因素,着重对比了实验动物不同物种与人类血液黏稠度、血液流速、血液 pH 值、体温的差别及对 FFA 显影强度及初次显影时间、维持时间、消退时间的影响。 同时分析了对不同实验动物物种间 FFA 差异和 FFA 拍摄时引起动物不良反应的因素。 希望提供这些信息和数据能够帮助解决研究者在实验动物 FFA 造影中遇到的问题,获得更准确可靠的检测结果,实现 FFA 检测在实验动物中的应用价值。

关键词: FFA, 显影强度, 初次显影时间, 消退时间, 显影时长, 实验动物